Research Experience-1

Aug 2012-Jan 2018

Doctor of Philosophy (Sc.), Microbiology

Affiliation: University of Calcutta, Kolkata, West Bengal, India

Work Place: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37 SP Mukherjee Road, Kolkata-700026, West Bengal, India

Advisor: Dr. Chinmay Kumar Panda

Title of the Thesis: Analysis of Regulation of Self Renewal Pathways and EGFR Expression in Uterine Cervical Carcinoma of Indian Patients

• The primary aim was to analyze the alterations of key genes involved in self-renewal (Hedgehog and Wnt) pathways and EGFR regulation during development of Uterine cervical carcinoma (CACX) in Indian patients (N=155). Molecular alterations of different regulatory genes suggested a plausible cross talk among of the Hedgehog, Wnt and EGFR pathways in development of CACX.

• The following techniques were used to address the research questions:

1. Microdissection, Tumor cell enrichment and genomic DNA Isolation from patient samples.

2. Copy Number variation analysis by CGH-SNP microarray.

3. Microsatellite markers based deletion analysis by PCR followed by denaturing PAGE.

4. Chromosomal amplification analysis by quantitative PCR.

5. Mutation analysis by SSCP analysis followed by Sanger Sequencing.

6. Promoter methylation analysis by PCR based MSRA and MSP analysis.

7. Total RNA isolation and mRNA expression analysis by qRT-PCR.

8. Immunohistochemistry (IHC) analysis for over or under expression of protein.

9. Cell culture and maintenance of HeLa, SiHa and HEK293Tcell lines.

10. Hemocytometer cell count, Trypan Blue Cell viability and Sulforhodamine B (SRB) assay.

11. In vitro validation of promoter methylation using 5-aza-dC followed by Immunocytochemistry.

12. Cloning, Transfection and Luciferase assay for promoter activity.

13. Western blot analysis.

14. Bioinformatics and Statistical analyses.

• Further involved in Expression microarray and DNA methylome analysis of Uterine Cervical Carcinoma of Indian Patients

Research Experience-2

Dec 2011-July 2012

Junior Research Fellow (Project), Department of Science and Technology, Ministry of Science and Technology, Govt. of India 

Work Place: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37 SP Mukherjee Road, Kolkata-700026, West Bengal, India

Advisor: Dr. Chinmay Kumar Panda

Project Title: Identification of tumor suppressor genes loci in chromosomes 3, 4 and 11 associated with the development of uterine cervical carcinoma

• Genetic alterations are the dominating event in the development of uterine cervical carcinoma. High-resolution whole genome CGH-SNP array showed the association of different tumor suppressor genes are situated in chromosomes 3, 4 and 11, and are associated with CACX development.

• The following techniques were used to address the research questions:

1. Microdissection, Tumor cell enrichment and genomic DNA Isolation from patient samples.

2. Copy Number variation analysis by CGH-SNP microarray.

3. Validation through deletion, promoter methylation, qPCR and expression analyses.

Research Experience-3

May 2011-Aug 2011

Summer Research Fellow, Bioinformatics Center

Work Place: Bose Institute, Kolkata-700054, India

Advisor: Prof. Tapas Chandra Ghosh

Project Title: Evolutionary Studies on Human Genome by Considering Different Protein Diversity Repertoire and Protein-Protein Interaction Network

• Alternative splicing and gene duplication are two major sources of proteomic function diversity. In this study we try to understand their effect on the evolutionary rate. Our observation revealed that evolutionary rate has the negative correlation with alternative splicing and gene duplication and both of them are negatively correlated with each other.

• The following techniques and databases were used to address the research questions:

1. Gene related informations were retrieved from Ensembl release 63 and EBI-EMBL website.

2. Protein related informations were retrieved from Human Protein Reference Database.

3. Microarray related data were downloaded from Human U133A/GNF1H Gene Atlas.

4. Statistical analyses were performed by using the SPSS (13.0).

Research Experience-4

June 2009-Aug 2009

Summer Research Fellow, Department of Oncogene Regulation

Work Place: Department of Oncogene Regulation, Chittaranjan National Cancer Institute, 37 SP Mukherjee Road, Kolkata-700026, West Bengal, India

Advisor: Dr. Chinmay Kumar Panda

Project Title: Identification of mutations in Exon18 of Epidermal Growth Factor Receptor gene in head and neck cancer by DNA sequence analysis

•   Mutation analysis of Exon 18-Intron 18 region of EGFR were studied in Head and neck squamous cell carcinoma (HNSCC) patients [N=20]. DNA sequence analysis 40% of the samples showed allelic variation (G>A) of the SNP rs17337107 located in the amplified region.

• The following techniques were used to address the research questions:

1. Genomic DNA was isolation.

2. Primer design using the online software Primer3 (v. 0.4.0)

3. PCR amplification followed by PCR product purification.

4. Big Dye Terminator Cycle Sequencing by using Applied Biosystem 3130xl Genetic Analyzer.

5. Chromatograms analyzed through Chromas Lite (Version 2.01) software and Clustal W [Version 1.81]. 

Teaching Experience-1

Sep 2011-December 2019

Guest Lecturer, Department of Microbiology

Vijaygarh Jyotish Ray College, University of Calcutta, Kolkata, India

Graduate and Undergraduate lecture and laboratory sessions in Molecular Biology, Recombinant DNA Technology, Genomics, Medical Biotechnology and Bioinformatics.

Teaching Experience-2

January 2020-Present

State Aided Colege Teacher (Category-I), Department of Microbiology

Vijaygarh Jyotish Ray College, University of Calcutta, Kolkata, India

Graduate and Undergraduate lecture and laboratory sessions in Molecular Biology, Recombinant DNA Technology, Genomics, Medical Biotechnology and Bioinformatics.